Job Description
Job Type: Consulting / Contract Function: Life Sciences R&D/Engineering Post Date: 01/10/2026 Website: www.positivobio.com Compensation: Competitive hourly/project-based rate About Positivo Biotechnology Positivo Biotechnology is a pioneering early-stage private company that is developing novel non-viral DNA gene delivery and expression systems to safely and effectively target various diseases including cystic fibrosis. We are seeking a skilled Protein Production Scientist Consultant to conduct laboratory studies for our non-viral gene delivery program. Position Overview The Protein Production Scientist Consultant will perform recombinant protein expression and purification using baculovirus and insect cell systems. This is a flexible consulting arrangement with scope and hours to be determined based on project needs. Key Responsibilities Baculovirus Expression System • Generate and amplify recombinant baculovirus stocks • Perform transfection and infection of Sf9 or other insect cell lines • Optimize expression conditions for target proteins Protein Purification • Purify recombinant proteins using chromatography techniques (affinity, ion exchange, size exclusion) • Perform protein analysis including SDS-PAGE, Western blot, and concentration determination • Maintain quality control standards for purified proteins Cell Culture • Maintain insect cell lines (Sf9) and/or mammalian cell lines following standard aseptic techniques • Scale up cell culture for protein production Documentation • Maintain accurate laboratory notebooks and experimental records • Prepare summary reports of experimental results Apply tot his job